2017; 02(04): 057-066 mds/16/1621/gmj

Original Research Article

Comparative Study of Natural Phenolic Acids and Flavonols as Antiplatelet and Anti-Inflammatory Agents

Alexandra Moschona1, Konstantinos D. Kyriakidis2, Athanassios D. Kleontas3, Maria Liakopoulou-Kyriakides1*
1* Faculty of Chemical Engineering, Section of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece.
2Colchester Hospital, University Foundation Trust, Turner Road, Colchester, Essex, CO45JL, UK.
3Department of Cardiothoracic Surgery, Aristotle University School of Medicine, AHEPA University Hospital, Thessaloniki, Greece.

Correspondence to
Maria Liakopoulou-Kyriakides, Faculty of Chemical Engineering, Section of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece.

First Published Online 02 May 2017


The search for natural phenolic compounds from fruits, vegetables, as well as from their wastes, has recently gained importance, due to their beneficial effects on human health and their potential use as drugs. Here, three phenolic acids (gallic, ellagic, ferulic) and two flavonols (quercetin and kaempferol), isolated from wine and pomegranate wastes, and the standards were evaluated based on their radical scavenging activity, antiplatelet and anti-inflammatory activity, in vitro. Specifically, the phenolic compounds were tested for their antioxidant activity, using the DPPH method, for their effect on human platelet aggregation induced, by collagen, ADP and subsequently against cyclooxygenases 1 and 2 activities. It was found that all phenolic compounds presented high antiradical activity and inhibited both collagen and ADP-induced platelet aggregation, in a dose-dependent manner with IC50 values ranging from 60.0±0.5 to 112±1.1 μΜ for collagen and ADP. Ferulic acid, gallic acid and quercetin found to exert significant effect on COX-1 enzyme with IC50 values at 15.0±0.5, 9.0±0.1, 15.0±0.4 μΜ, respectively and kaempferol at a moderate level (IC50 58.0±0.9 μΜ). Ferulic acid, gallic acid, and kaempferol also inhibited COX-2 enzyme with IC50 values at 4.0±0.1, 14.0±0.3, 54.0±0.9 μΜ, respectively. Interesting, ellagic acid did not show any effect on both COX enzymes, whereas quercetin presented only anti-COX-1 activity at the concentrations tested. The study demonstrated the multifunctional role of the above natural compounds with all presenting high antiradical, and antiplatelet activity, while, differences and interesting data were obtained with respect to their anti-COX activity. The data showed the potential use of the most active compounds in dietary foods and pharmaceutical industries.

Keywords: phenolic compounds, antiplatelet activity, anti-inflammatory activity, collagen, ADP, COX enzymes

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